Influence of Different Inactivation Methods on Severe Acute Respiratory Syndrome Coronavirus RNA Copy Number

Reverse transcription-quantitative PCR (RT-qPCR) is currently regarded as the gold standard for the diagnosis of SARS-CoV-2 infection. In China, nucleic acid assays of SARS-CoV-2 are required to be conducted in biosafety level 2 laboratories.

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Quantitative detection and viral load analysis of SARS-CoV-2 in infected patients

A novel coronavirus, severe acute respiratory syndrome coro-navirus 2 (SARS-CoV-2), has been identified as the pathogen of the coronavirus disease 2019 (COVID-19). We compared RT-PCR and ddPCR for COVID-19 diagnosis.

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Pitfalls in RET Fusion Detection Using BreakApart FISH Probes in Papillary Thyroid Carcinoma

Thyroid carcinoma is the most common endocrine cancer, of which papillary thyroid cancer (PTC) accounts for ap-proximately 80% to 90%. Notably, to our knowledge, the FISH false-negative cases in PTC observed in this study have barely been reported.

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Highly Sensitive Droplet Digital PCR Method for Detection of de novo EGFR T790M Mutation in Patients with Non-Small Cell Lung Cancer

Sensitizing mutations in the epidermal growth factor receptor (EGFR) gene are the most frequent oncogenic alterations in non-small cell lung cancer (NSCLC). Droplet digital PCR (ddPCR) is a new generation of PCR technique for the detection of EGFR.

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Establishment and validation of a novel droplet digital PCR assay for ultrasensitive detection and dynamic monitoring of EGFR mutations in peripheral blood samples of non-small-cell lung cancer patients

Lung cancer is the leading malignant tumor in mortality worldwide. Droplet digital polymerase chain reaction (ddPCR) is an emerging technology that could be employed in clinical areas, such as tumor, infectious disease, non-invasive prenatal testing.

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