The diagnosis was based on the Diagnosis and Treatment Protocol for COVID-19 (trial version 7) established by the National Health Commission of the People’s Republic of China.
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PCR is a molecular technique for exponential amplifica-tion of a specific segment of DNA, which was developed by Kary Mullis in the 1980s.
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Droplet digital polymerase chain reaction (ddPCR) achieves abso-lute quantitation and is highly sensitive. In ddPCR, template DNA molecules are distributed across numerous droplets, so that the number of template DNA molecules can be quantified.
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Coronavirus disease 2019 (COVID-19) is a new and emerging in-fectious disease that has spread rapidly across the globe. In this study, 117 clinical samples were analyzed qualitatively and quantitatively by RT-qPCR and droplet digital PCR (ddPCR).
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IMPORTANCE Convalescent plasma is a potential therapeutic option for patients with coronavirus disease 2019 (COVID-19), but further data from randomized clinical trials are needed.
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