Mycobacterium tuberculosis nucleic acid digital PCR detection kit
This kit is designed based on droplet digital PCR technology and TaqMan fluorescent probe technology to specifically detect Mycobacterium tuberculosis complex (MTBC) nucleic acid in human sputum samples.
Analytical Sensitivity (LOD): the kit detects 0.1% MTBC nucleic acid in human sputum samples.
Analytical Specificity (Cross-reactivity): testing on 200 ng of wild-type nucleic acid samples, the test result is negative; no cross-reactivity with nontuberculous mycobacteria, common respiratory viruses, respiratory pathogenic bacteria, etc.
Accuracy: accuracy of the kit was established by testing internal positive references and negative references, the detection rates are 100%.
Precision: precision of the kit was established by performing precision references for 10 repeats, all results were positive, and the coefficient of variation (CV, %) of the logarithm of copy numbers has been no more than 5%.