EGFR mutations digital PCR detection kit
The EGFR mutations digital PCR detection kit is designed based on droplet digital PCR technology and TaqMan fluorescent probe technology to specifically detect the mutations of EGFR gene in cell free DNA from plasma samples and formalin-fixed paraffin-embedded (FFPE) tissue DNA of patients with non-small cell lung cancer.
Performance Characteristics
Sample types: FFPE (Formalin-Fixed, Paraffin-Embedded) tissue, plasma.
Analytical Sensitivity (LOD): the kit detects 0.1% of human EGFR gene mutations in plasma samples, and 0.3% of human EGFR gene mutations in FFPE tissue samples.
Analytical Specificity (Cross-reactivity): testing on 200 ng of wild-type nucleic acid samples, the test result is negative; no cross-reactivity.
Accuracy: accuracy of the kit was established by testing internal positive references and negative references, the detection rates are 100%.
Precision: The coefficient of variation (CV, %) of the logarithm of copy numbers has been no more than 5%.
